Host species

E.coli

Source

Prokaryotic expression

Residues

Ala965~Arg1303

Subcellular Location

Secreted

Tags

N-terminal His Tag

Purity

> 90%

Buffer formulation

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose

Traits

Freeze-dried powder

Predicted isoelectric point

5.9

Predicted Molecular Mass

41.6KDa

Accurate Molecular Mass

42kDa as determined by SDS-PAGE reducing conditions

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not

vortex.

Storage instructions

Stable for 1 year at -80℃.

Aliquot to avoid repeated freezing and thawing.

Store at 2-8℃ for one month.

For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.