Source

Prokaryotic expression

Host species

E.coli

Residues

Met1~Asp103 linked with Leu142~Gln187

Tags

N-terminal His Tag

Appearance

Freeze-dried powder

Size

50μg

Purity

> 95%

Buffer formulation

 PBS, pH7.4, containing 0.01% SKL, 5% Trehalose

Predicted isoelectric point

8.3

Accurate Molecular Mass

18.7kDa as determined by SDS-PAGE reducing conditions

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage instructions

Stable for 1 year at -80℃.

Aliquot to avoid repeated freezing and thawing.

Store at 2-8℃ for one month.

For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence