Source

Prokaryotic expression

Host species

E.coli

Residues

Ile229~Val465

Tags

N-terminal His Tag

Subcellular Location

Nucleus, Mitochondrion, Cytoplasm, Endoplasmic reticulum lumen

Appearance

Freeze-dried powder

Purity

> 80%

Buffer formulation

100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.

Original Concentration

300μg/mL

Predicted isoelectric point

6.5

Predicted Molecular Mass

30.4kDa

Accurate Molecular Mass

30 kDa as determined by SDS-PAGE reducing conditions.

Usage

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage instructions

Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

Sequence