Source

Prokaryotic expression

Host species

E.coli

Residues

Trp18~Leu275

Subcellular Location

Secreted

Tags

N-terminal His Tag

Appearance

Freeze-dried powder

Original Concentration

120μg/mL

Purity

> 90%

Buffer formulation

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose.

Predicted isoelectric point

8.2

Accurate Molecular Mass

33kDa as determined by SDS-PAGE reducing conditions.

Predicted Molecular Mass

32.7KDa

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage instructions

Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence