Source

Prokaryotic expression

Host species

E.coli

Residues

Thr37~Leu340

Tags

N-terminal His Tag

Subcellular Location

Secreted

Appearance

Freeze-dried powder

Purity

>95%

Buffer formulation

PBS, pH7.4, containing 0.05% SKL, 1mM DTT, 5% Trehalose and

Proclin300

Predicted isoelectric point

6.3

Predicted Molecular Mass

37.4kDa

Accurate Molecular Mass

37kDa as determined by SDS-PAGE reducing conditions

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage instructions

Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

Sequence