Source

Prokaryotic expression

Host species

E.coli

Residues

Ser21~Ala99

Tags

Two N-terminal Tags, His-tag and SUMO-tag

Endotoxin Level

 <1.0EU per 1μg (determined by the LAL method). 

Purity

> 95%

Buffer formulation

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose

Predicted isoelectric point

5.8

Accurate Molecular Mass

21kDa as determined by SDS-PAGE reducing conditions

Predicted Molecular Mass

22.5KDa

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage instructions

Stable for 1 year at -80℃.

Aliquot to avoid repeated freezing and thawing.

Store at 2-8℃ for one month.

For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence