Source

Prokaryotic expression

Host species

E.coli

Residues

Ala2~Ser251

Subcellular Location

Cytoplasm

Tags

N-terminal His Tag

Appearance

Freeze-dried powder

Size

10 μg

Purity

> 90%

Buffer formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Predicted isoelectric point

5.6

Predicted Molecular Mass

31.8kDa

Accurate Molecular Mass

34kDa as determined by SDS-PAGE reducing conditions.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage instructions

Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

SEQUENCE