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Recombinant Beta Catenin (β-catenin)说明书

2024-03-22

Recombinant Beta Catenin (β-catenin)

Organism Species:Homo sapiens (Human)

Applications:Positive Control; Immunogen; SDS-PAGE; WB.

 

 PROPERTIES

Source

Prokaryotic expression

Host species

E.coli

Residues

Cys439~Ser675

Subcellular Location

Nucleus, Cytoplasm

Tags

N-terminal His Tag

Appearance

Freeze-dried powder

Size

10 μg

Purity

> 97%

Buffer formulation

PBS, pH7.4, containing 0.01% SKL, 5% Trehalose .

Predicted isoelectric point

7.0

Predicted Molecular Mass

27.7kDa

Accurate Molecular Mass

27kDa as determined by SDS-PAGE reducing conditions.

Usage

Reconstitute in ddH2O to a concentration less than or equal to 0.1mg/mL. Do not vortex.

Storage instructions

Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months

Stability Test

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

SEQUENCE

CQ

VGGIEALVRT

VLRAGDREDI

TEPAICALRH

LTSRHQEAEM

AQNAVRLHYG

LPVVVKLLHP

PSHWPLIKAT

VGLIRNLALC

PANHAPLREQ

GAIPRLVQLL

VRAHQDTQRR

TSMGGTQQQF

VEGVRMEEIV

EGCTGALHIL

ARDVHNRIVI

RGLNTIPLFV

QLLYSPIENI

QRVAAGVLCE

LAQDKEAAEA

IEAEGATAPL

TELLHSRNEG

VATYAAAVLF

RMSEDKPQDY

KKRLS